Speaker: Prof. Henning Jessen, University of Freiburg
Title: Tools to Study the Function of Inositol Pyrophosphates in Living Cells
Location: 408/Building 24
Time: 11.00 am on On Friday, 1 April
Tools to Study the Function of Inositol Pyrophosphates in Living Cells
Diphosphoinositol polyphosphates (X-PP-InsP5) have been found in all domains of biology. They play important roles in different biological processes, such as energy homeostasis, toxin deactivation, stress responses, signal transduction, cancer development and insulin release, but how they regulate such diverse processes is poorly understood. It has been proposed that 5-PP-InsP5 might regulate proteins by controlling their intracellular localisation. Here, we demonstrate that the controlled intracellular release of 5-PP-InsP5 from a photocaged precursor leads to translocation of the PH domain of the kinase Akt from the membrane into the cytoplasm. Such technologies are required to understand inositol pyrophosphate signalling in more detail than previously possible.